Regulatory

Part:BBa_K112318:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-21)


{< bolA promoter>} in BBb format


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR al0017/al0018 on MG1655     (467bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112318
-----------------------------------------------
al0017	Forward Biobricking of bolA promoter
CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc
al0018	Reverse Biobricking of bolA promoter
CGttaGGATCCcttctatccgctcacgtatc



Source

MG1655

References